In this paper was presented a scarce incident of presence the triungulin larvae of Meloe proscarabeus L. in the honeybee colony. The invasion of the beetles larvae from Meloidae were quoted only in aged beekeeping papers. As well as, a parasite of the honey bee was recognize only larvae of Meloe variegatus (Donow.), which were feed on the honeybee’s haemolymph. The present event related to larvae of Meloe proscarabeus in the honeybee colony. The larvae of this beetle species were dragged to the bees colony, in which were make the life cycle, but letter were died. In the honeybee colony the larvae of Meloe proscarabeus nourish on the stored food and the bee’s eggs. The damages caused to the larvae were inconspicuous.
THE INFLUENCE OF Β-ADRENERGIC RECEPTORS ON UTERUS MOTILITY IN THE RAT IN IN VITRO RESEARCH
Autor
Albert Czerski, Maciej Janeczek, Edyta Wincewicz, Marek Włodarczyk, Marcin Zawadzki, Wojciech Zawadzki
Pages
9–18
Keywords
uterus motility, rat, β-adrenergic receptor
Abstract
Show abstract
The experiments were carried out on 15 rats aged between 4–6 months. Myometrial tissue (1,5– 2 cm long) was set up in 4 isolated organ baths (20 ml) at 37 oC. After recording the pattern of myometrial tissue spontaneous motility: salbutamole –β2-adrenergic receptors agonist, dobutamine –β1-adrenergic receptors agonist, propranolol –β1 – and β2-adrenergic receptors antagonist, isoprenaline –β1 and β2-adrenergic receptors agonist, adrenaline –α and β-adrenergic receptors agonist were added into the organ baths. The dose of the formulae was fixed in preliminary experiments. Adrenergic receptor activated by salbutamole selective agonist caused decrease in myomerial motility. On the other hand adrenergic receptor blocked by propranolol eliminated the restraining influence of salbutamole on myometrial motility. Adrenergic receptor activated by dobutamine selective agonist caused decrease in uterus motility. Adrenergic receptor blocked by propranolol antagonist eliminated the restraining influence of dobutamine on uterus. Adrenergic receptor activated by isoprenaline and adreneline also caused decrease in uterus motility. The experiments exhibited the presence of both the adrenergic receptor subtypes (β1 and β2) in the myometrial of rats. Adrenergic receptor activated by β-adrenergic agonist caused decrease in uterus motility.
THE STRUCTURE AND TOPOGRAPHY OF GYRUS PARAHIPPOCAMPALIS IN THE POLAR FOX (ALOPEX LAGOPUS)
Autor
Ryszard Eustachiewicz, Radosław Szalak, Iwona Łuszczewska-Sierakowska
Pages
19–28
Keywords
central nervous system, limbic system, gyrus parahippocampalis, polar fox (Alopex lagopus)
Abstract
Show abstract
The examination was carried out on 5 brains of sexually nature in the polar fox (Alopex lagopus). The material of the examination after fixing, dehydrating ,embedding in paraffin and cutting was coloured according to Klüver and Barrer`s method. Gyrus parahippocampalis which was the object of examination is a cortical structure which joins formation hippocampus with neocortex. It is a rear part of gyrus fornicatus which stretches from splenium of corpus callosum to the abdomino- medial angle of cerebral hemisphere. It is` made up of the following cortical structures: area entorhinalis, parasubiculum and presubiculum. The above mentioned parts of gyrus parahippocampalis` cortex are made up of four layers: marginal, cellular I, cellular II, and cellular III.
MORPHOLOGICAL CHARACTERISTICS OF AMERICAN STAFFORDSHIRE TERRIER BREED (AST) IN DOLNY ŚLĄSK AND WIELKOPOLSKA ON THE BASIS OF GIVEN MORPHOMETRIC PARAMETERS
Autor
Aleksander Chrószcz, Maciej Janeczek, Norbert Pospieszny, Monika Wojnar
Pages
29–35
Keywords
American staffordshire terrier, biometry, dog
Abstract
Show abstract
Biometry is a crucial criterion useful in determining of sexual dimorphism and in dog’s eksterier dog breed that was introduced in Poland in 1989 [Jurek 1993, Jurek1999, Kuchlewski 1999]. Since then we have experienced a dynamic growth of this breed population. In this paper an attempt to create a morphological characteristics of these dogs breed population in Dolny Śląsk and Wielkopolska. Biometric parameters allowing the optimal valuation of crucial and specified in the breed model features were chosen.
The nine cases of the canine tooth fractures in dogs was described. All fractures were qaualifited as a fracture with breakthrough line in contact with dental pulp. The complete root canal therapy was carried out in all cases. The root canals were fillied with preparate on a base of the zinc oxide and eugenol. The root canals were closed with sandwitch method using glass-ionomer and then composite. The treatment was procceded in general intavenous anaesthesia.
LEUKOCYTES FUNCTION IN THE COURSE OF TUMOUR PROGRESSION IN A RAT MODEL
Autor
Stanisław Graczyk, Bartłomiej Janaczyk, Janusz A. Madej, Marcin Nowak, Aleksandra Pliszczak-Król
Pages
45–51
Keywords
transplanted tumours, leukocytes, phagocytosis, radial segmentation of lymphocytes, rats
Abstract
Show abstract
Wistar rats, aged 2,5 months, were intraperitoneally inoculated with the cells of three model tumours: Yoshida sarcoma, Morris hepatoma and mammary carcinoma. On the 6th day after inoculation the phagocytic potential of the neutrophils (in a mixture with yeast cells – Saccharomyces cerevisiae) was evaluated. Simultaneously, the percentage of lymphocytes with nuclei, undergoing the radial segmentation (RS) - spontaneous or induced with oxalate mixture was determined. It was shown that in experimental rats, the change of leukocytes number and proportion change of particular leukocytes forms are accompanied by the weakening of phagocytic potential of the neutrophils. There was also noted an increase of the number of lymphocytes undergoing the spontaneous RS, while the number of the lymphocytes with the RS induced by the oxalates decreased. The results obtained indicate that the leukocytes changes depend in large measure on the tumour progression degree.
THE CORRELATION OF A SKIN pH OF DOGS WITH THE PYODERM TREATMENT BEFORE AND AFTER USAGE OF SURFACE-ACTING AGENTS OF KNOWN pH REACTION
Autor
Józef Nicpoń, Jarosław Popiel
Pages
53–60
Keywords
dogs, pyoderma, skin pH
Abstract
Show abstract
The goal of the work is to establish the values of skin pH of dogs in the physiological state and in the pathological state as pyoderma is. The research was made on 40 adult dogs; 20 healthy and 20 with skin changes. From the changed places of skin the microbiological smears were taken and pH of the skin in those places was measured. The dogs with pyoderma were treated with surface-acting agents as shampoos of known pH reaction. Before and after the treatment the pH of the skin was measured. It was noticed that the pH of the dog skin is higher than human and other carnivores; it was established as pH 7,395. It was discovered that in the pyodermal states the skin pH is elevated up to 8,109. The usage of a surface-acting agents caused normalization of a skin pH.
THE PRELIMINARY RESEARCH CONCERNING MICROBIOLOGICAL IMAGE, MORPHOLOGY AND SOME DIGESTIVE TRACT FERMENTATION PARAMETERS OF RATS
Autor
Albert Czerski, Katarzyna Płoneczka, Edyta Wincewicz, Wojciech Zawadzki
Pages
61–69
Keywords
rat, bacteria, morphology, fermentation, digestive tract
Abstract
Show abstract
The experiments were carried out on 24 Bufflo rats (12 male and 12 female). The animals were fed ad libitum with LSM granules for rodents. Access to water was unlimited. After the end of the experiments the rats were put to ether narcosis, while smear tests from mouth and pharynx were done and then blood from jugular vein was taken. After putting to sleep stomach and caecal were withdrawn and then their contents. The withdrawn contents were cultured on the bacteriological ground. The number of erythrocytes and leucocytes as well as haemoglobin and haemocrit were marked in blood. Glucose, total protein, total cholesterol and triglycerides were marked in blood serum. Artificial saliva and distilled water in equal amounts were added to the stomach contents. Eight-hour contents incubation was conducted in special vessels located in an automatic water bath with a temperature of 39 ºC, during which samples for gas, ammonia, pH analysis were taken. The most frequently isolated bacteria was Escherichia coli which was found in smear tests from ileum in 95,8% and from caecal in 83,3% of the examined rats. Bacillus sp. was largely isolated from caecal (95,8%) quantities. Bacillus sp. was largely isolated from caecal (95,8%). The conducted experiments indicated that ammonia contents is higher in caecal than in stomach. Substantial differences connected to sex are also exhibited. The same case is with the lactic acid level. The pH level is lower in stomach (3,8 to 4,1), and in caecal it is 6,8.
The respiratory tract of reptiles is anatomically and phisiologically very different from that of mammals. Snake lungs are elongated and consist of anterior portion-respiratory lung and posterior portion – saccular lung. In most (85%) snakes, the left lung is absent. In the boas and pythons and anaconda have two lungs. The right lung is longer than the left. Morphology in the lungs of the boidae is very interesting.
THE LEVELS OF SELECTED BIOCHEMICAL INDICES IN BLOOD OF THE RATS INJECTED WITH VITAMINS A AND D3 AND EXPOSED TO OZONE
Autor
Karol Jakubowski, Maria Jedlińska-Krakowska, Andrzej Kowalski
Pages
79–84
Keywords
rats, ozone, vitamin A, vitamin D, glucose, total protein, total lipids, triglycerides, alkaline phosphatase
Abstract
Show abstract
The levels of glucose, total protein, total lipids, triglicerides and activity of alkaline phosphatase were measured in rats injected with different doses of vitamins A and D3 and kept in atmosphere of elevated level of ozone in air. Investigations showed that mentioned vitamins did not protect the animals against long term exposition of ozone. However, vitamines used in higher doses caused decreased the levels of total proteins and activity of alkaline phosphatase and increased the concentration of total lipids.
THE INFLUENCE OF THE DIET BASED ON HAY AND C-J MIXTURE WITH ADDITION OF THE PROTEIN-MINERAL SUPPLEMENT ON THE SELECTED SHEEP BLOOD PARAMETERS
Autor
Albert Czerski, Jarosław Hreczyński, Adam Malicki, Wojciech Zawadzki
Pages
85–90
Keywords
sheep, livex, hematological index
Abstract
Show abstract
The experiments were carried out on 24 inter-breed hybrid sheep 24 to 50 months old and of the body mass between 38-45kg. The animals were divided into two groups. The first group consisted of 12 sheep fed on meadow hay (ad libitum) and C-J mixture (100g per animal). The second group was fed on meadow hay (ad libitum) and C-J mixture (100 g per animal) with brown livex supplement dosed 0,5 g per one kg of the body mass. Water was given ad libitum. After 4 weeks blood was taken for examination. The number of erythrocytes and leukocytes as well as haemoglobin and haemocrit was marked in the blood. Total protein, Fe2+, Ca2+, Mg2+ was marked in the blood serum. Livex supplement caused a statistically significant increase in Haemoglobin, Fe2+, Mg2+, Cu2+ and leukocytes 13, 10, 34, 12 and 22% respectively. An increase in erythrocytes, haemocrit and total protein was also noticed, but it was small and therefore statistically insignificant. The results exhibit how beneficial livex is as the fodder supplement in the sheep feeding.
COMPARISON OF THE MICROBIOLOGICAL DURABILITY OF PATES STORED UNDER THE REFRIGERATION
Autor
Szymon Brużewicz, Adam Malicki
Pages
91–97
Keywords
pates, moulds, water activity
Abstract
Show abstract
Contamination with pathogens and short microbiological durability are the main hygienic problems related to pates. The purpose of the studies was the durability comparison of two different pates stored under the refrigeration. We also tried to determine which of the intraenvironmental factors have the strongest impact on the microbiological status of the material studied. Both the pates were stored for 10 days. Microbiological parameters, water activity and pH values were measured at the experiment days 0, 3, 7 and 10. The pathogens were not isolated from any sample in course of the entire experiment. The total plate count in the pates increased by 0,4–0,5 log CFU x g-1 after 10 days of storage, and consequently the threshold of bacterial spoilage was not exceeded. The moulds were isolated from the material studied from 3rd day of experiment, with the higher counts determined in poultry pate. External water activity seems to be the factor affecting the mould growth within the material studied.
SUPEROVULATION AND TECHNICS OF COLLECTION AND TRANSFER OF EMBRYOS IN SWINE RECOVERY
Autor
Paweł Antosik, Jędrzej M. Jaśkowski
Pages
99–105
Keywords
superovulation, surgically and non-surgically methods of embryo collection, embryo transfer, swine
Abstract
Show abstract
Some aspects of embryotransfer in sows were discussed. The heat synchronization using altrenogest and superovulation models with PMSG or FSH and hCG and its effectivenes were characterised. It shows surgically and non-surgically methods of embryo collection in swine with especially regards on endoscopically embryo flushing. New develop non-surgically procedure of embryo deep intrauterine transfer to recipients sows using modified flexible catheter were descirbed.
SURGICAL TREATMENT FOR SOME MUSCULO-TENDINOUS DISEASES OF THE SHOULDER IN DOGS
Autor
Zbigniew Adamiak, Rafał Chorosiński, Piotr Holak
Pages
107–113
Keywords
dog, mineralization of musculus supraspinous, inflammation of tendom of musculus biceps brachium
Abstract
Show abstract
The presented study described 5 dogs with shoulder musculo-tendinous diseases. 4 dogs had bicipital tenosynovitis, and one dog had tendon mineralization of supraspinatus muscle. In all dogs surgical procedures were performed. In dog with mineralization changes on supraspinatus muscle surgically debulking mineralized mass treatment was employ. In four dogs with bicipital tenosynovitis tenodesis was performed. In all cases clinical improvement was excellent, surgical therapy successfully results in alleviating discomfort, and all dogs returned to normal activity.
The purpose of the study was to determine the influence of environment on concentration of chromium and nickel in tissues of hunting animals (roes and wild pigs), coming from two different in consideration of contamination regions (Wrocław and Legnica). The study was made in 2003. Liver, kidneys, muscles and hair samples were taken and mineralized dry a muffle furnace at 450 ºC. Metals were determined directly in mine-ralizate by the ICP –AES method on the Varian apparatus. Results of the study indicate on significant influence of the city environment on chromium and nickel concentration in tissues of hunting animals. It was confirmed by higher levels of nickel and chromium in hairs of roes and wild pigs coming from Wrocław.
The aim of the study was to investigate how inhibition of the converting enzyme activity would affect the concentration of potassium and magnesium in the plasma and erythrocytes of calves in their early neonatal age. The studies were carried out on 10 Black-and-White calves over the first seven days of their life. In order to block the angiotensin I converting enzyme, the calves was administered per os 0.2 mg·kg-1 b.w. of captopril (Captopril – Jelfa). The results suggests that captopril administration results in increasing potassium and magnesium concentrations in calf blood plasma and in reduction of these electrolytes levels in erythrocytes. The ratio of potassium concentration inside erythrocytes to blood plasma potassium concentration after captopril administration changed only slightly, like the ratio of magnesium concentration inside erythrocytes to that in blood plasma.