Indole-3-carbinol (I3C) is a plant metabolite produced in the cruciferous vegetables including cabbage, Brussels sprouts and cauliflower. This agent has proved chemoprotective against chemically induced carcinogens, blocking the initiation of tumors in the rat tissues, including liver, colon and mammary gland. The mechanism of this chemoprotective effect is not entirely understood, but has probably been linked to the induction of detoxification enzymes, including phase I enzymes (cytochrome P-450-dependent monooxygenases) and phase II enzymes, such as glutathione S-transferase (GST; EC 2.5.1.18). Therefore it is suggested that consumption of the diets rich in cruciferous vegetables has been associated with the decreased risk of cancer. The effect of I3C on lipid metabolism has not been evaluated, so far. The experiment was conducted on 24 male Wistar rats. The animals were assigned randomly into 3 groups (n=8). Diet and drinking water were available ad libitum. The control group was given vehicle alone by oral gavage in dose 1.5 ml /100g body weight. Indole-3-carbinol was given daily in doses: low 50 mg/kg b.w. and high 150 mg/kg b.w. by oral administration during 7 days. The serum and tissues were used for the estimation of lipid parameters and detoxification enzymes. The results of the present study demonstrate enhancing effect of I3C on induction of hepatic GST. This agent caused a significant increase of GST activity (p ≤ 0.01). Glutathione peroxidase (GPx; EC 1.11.1.9) activity was negligible decreased. Total cholesterol concentration was significantly increased (p ≤ 0.01) in liver only. Other lipid indices in serum and liver have shown slight changes.

Investigation was aimed to choose the optimal freezing technique and to estimate the effect of addition of dimer of lysozyme on the quality of deep frozen boar spermatozoa. The investigations were carried out on 115 ejaculates. Semen was frozen in large, flat straws and pellets. The highest quality of boar semen after thawing was observed in semen processed and frozen according to Pursel and Park method in the form of pellets and flat straws. Significantly higher motility and intact acrosomes was observed in the samples where 4 and 6 μg dimer of lysozyme was added.

The research was conducted on 6 inter-breed hybrid sheep, at the age of 24 to 50 months and of the body mass of 38-45 kg, which had bipolar electrodes implanted to uterus horns and shank. The registration of uterus myoelectric activity was performed on sheep 24 and 48 hours after sensitizing with estradiol in dose 1,25 mg/on animal. This study showed that there is no statistical differences between uterus horns and shank in myoelectrical activity. The obtained data of the myoelectrical index (MI) in sheep in anestrus 24 hours after sensitizing with estradiol, were MI=10,07±2,85%/h on shank, MI=9,95±2,99%/h on right horn and MI=8,54±2,58%/h on left horn. The frequency of cycles (spindles) of action potentials 24 hours after sensitizing with estradiol were 1,38±0,30/ min. on shank, 1,45±0,29/min on right horn and 1,49±0,26/min on left horn. The value of myoelectrical index 48h after sensitizing were MI=14,77±2,6%/h on shank, MI=13,41±2,53%/h on right horn and MI=12,86±2,85%/h on left horn. The frequency of cycles of action potentials 48 hours after sensitizing with estradiol were 2,07±0,31/min on shank 2,00±0.29/min on right horn and 1,98±0,25/min on left horn. After 48-hours estradiol sensitivisation, as compared to 24-hours estradiol sansitivisation, MI and frequency of cycles of action potentials increased, respectively, by average 4,2%/h and 0,57/min. There were no statistically essential differences found between shank and horns activity. The result of this research is the following: uterus horns and shank both react likewise to estradiol sensitivisation and show similar myoelectrical activity after such stimulation.

Investigation was made passes on 12 Persian cats. Fetuses to arises from perinatal period. It was done morphological analysis of the aorticorenal ganglion, in support to the morphometry and morphologicalstructure. It was the nervous conjunction aorticorenal ganglion with associates.

The total serum LDH activity was determined in beef and dairy cows. LDH was also divided into isoenzymatic fractions. The total activity of lactate dehydrogenase was the highest in Limousine cows, and the lowest – in H-F ones. 5 LDH isoenzymes were present in all of the breeds examined. The activity of fractions LDH1 and LDH2 was higher in dairy cows, whereas the activity of fractions LDH3, LDH4, and especially LDH5 – in beef ones.

Experiments was carried out with mice of CFW infected with 200 T. pseudospiralis. The animals were killed between 7–365 days days post infection and masseter muscles were sectioned in a cryostat and examined using histochemical methods or immunoenzymatic and immunofluorescent methods with monoclonal antibodies. The composition of cells infiltration in the muscle were observed: mast cells, eosinophils, B lymphocytes, plasma cells, CD4⁺ lymphocytes and macrophages. The percentage composition of the cells in inflamatory infiltration in masseter muscles was as follows: eosinophils and CD4⁺ lymphocytes were the most numerous, but the percentage of mast cells, plasma cells, B lymphocytes and macrophages was a little.

Investigation was made on 4 sheeps, 3 male and 1 female, the fetuses coming from 2 uteruses. The age sheeps was evaluated at 5^th month of gestation. The morphological analysis of the lacrimal gland was made, with discription of site lacrimal gland on sclere, as well as distance between lacrimal gland and groove sclere.

The purpose of the study was to determine the changes in the distribution of „endogenic” iron in rabbit tissue and body fluids after the administration of ditiocarb sodium (DTC).The study was conducted on clinically healthy mongrel rabbits. Two groups were administered DTC intravenously: the first one at the low dose of 20 mg/kg b.w. and the latter at the high dose of 200 mg/kg b.w. The control group was animals administered saline solution ( sodium chloride). After euthanasia ,in 72^nd hour after injection of DTC samples of liver, kidneys, brain, skeletal muscles, perirenal fat, urine and blood were taken and mineralised dry in a muffle furnace at 450 0C. Iron was determined directly in mineralisate by the atomic spectrophotometry on a Pye Unicam SP-9 apparatus. The results obtained were subjected to statistical analysis by the Student t-test. Animals given low dose of DTC were having statistically related decrease of iron in fat, urine and plasma,and unrelated in liver,muscle and blood. After the administration of high doses of DTC (200 mg/kg b.w.) „endogennic” iron level were having statistically related increase in kidneys and unrelated in brain,liver, urine and plasma. In addition, it has been found, statistically relevant ,decrease iron level in adipose. The results of the study indicate that DTC administered intravenously in rabbits at the dose of 20 or 200 mg/kg b.w. leads to the substantial redistribution of iron in tissues and body fluids.

The aim of the present work was a detection of bacterial antigen in carps liver, kidney and spleen . Fish were immunised intra peritoneal by Aeromonas hydrophila bacterial antigen. 60 carps were divided into two experimental groups (I and II) 30 carps from the group I were injected 5x10⁷ml^–1 killed bacterial suspension, 30 from group II injected i. p. with the same bacterial suspension with adjuwant Emulsigen. Samples were taken for immunohistochemistry after 12, 72 hours, 1 and 2 weeks and after 1 and 3 months. We have shown that this method allowed to early detection of bacterial antigens in internal organs samples.

The objective of this study was comparative histological evaluation of healing of intestinal anastomoses in pigs operated on with different methods, i.e. mechanical and manual anastomoses. We decided to trace the process of healing of the intestinal wall anastomosed with biofragmentable anastomosis ring (BAR, Valtrac), round stapler (ILS 25) and manually placed nonabsorbable polyester sutures (Mersilene). The healing of the anastomoses was evaluated histologically in 21 pigs, on the 14^th, 30^th and 90^th day after the operation. The healing of anastomoses made with different techniques and materials was evaluated based on the score of overall intensity of inflammatory reaction proposed by Sewell and Deveney. It was found that the inflammatory reaction in the intestinal wall was least pronounced after the use of BAR. The anastomoses made with polyester sutures and steel staples produced more intensive inflammation in the first phase, due to the presence of a foreign body.

12 pigs underwent the experiment. The animals were all sexually mature. In the group of the experimental animals – in 9 pigs – the experimental procedure was performed which depended on lateral or bilateral extirpation of the specific complexes of the mammary gland with leaving the skin. The remaining pigs constituted the control group. All animals were kept alive for 21 days, after which they were put to sleep and the following material was taken for further examination: the brain stem, the thoracic-lumbar-sacral part of the spinal cord with adequate nerve ganglia, sympathetic trunk ganglia and the following ganglia and plexuses of abdominal and pelvic cavity: celiac, cranial mesenteric, intermesenteric, caudal mesenteric, hypogastric and pelvic. The material was elaborated on histologically. The experiments conducted on experimental and control animals caused the occurrence of deep degenerative lesions in the nerve cells of the central and the peripheral nervous system, which gave rise to establishing of the localization of the sympathetic nerve cells innervating the mammary gland in pig. The results of the study and the localization of the retrograde changes allow stating that the sympathetic nerve cells reaching the mammary gland are of multi-source origin. They come both from the central and peripheral nervous system.

8 female dogs underwent the experiment. The animals were all sexually mature. In the group of the experimental animals – in 7 animals – the experimental procedure was performed which depended on lateral or bilateral extirpation of the specific complexes of the mammary gland with leaving the skin. 1 to 3 complexes were extirpated (anterior, medial, or posterior). The remaining one constitued the control group. All animals were kept alive for 21 days, after which they were put to sleep and the following material was taken for further examination: the brain stem, the thoracic-lumbar- sacral part of the spinal cord with adequate nerve ganglia, sympathetic trunk ganglia and the following ganglia and plexuses of abdominal and pelvic cavity: celiac, cranial mesenteric, intermesenteric, caudal mesenteric, hypogastric and pelvic. The material was elaborated on histologically. The experiments conducted on experimental and control animals caused the occurrence of deep degenerative lesions in the nerve cells of the central and the peripheral nervous system, which gave rise to establishing of the localization of the nerve cells innervating the mammary gland in female dog. The results of the study and the localization of the retrograde changes allow stating that the nerve cells reaching the mammary gland are of multi-source origin. They come booth from the central and peripherial nervous system.

The effect of training, age and sex of Arabian horses on blood lactate dehydrogenase (LDH) activity was investigated. 69 horses were observed in age 3–9 years trained before starts on the racetrack. The level of LDH at rest was the higher in blood of fillies, lower in group of colts and the lowest in older stallions. In all groups increase in LDH after exercise was observed. It was on this same level over 30 min after endurance work and went down after race training.

The authors examined in vitro the influence of the ES antigen of T. spiralis on the cells of lymphatic nodes in healthy mice. They found that this antigen is capable of causing apoptosis. The number of the cells demonstrating such changes depends on the concentration of the antigen and on the time of reaction. After 3 hours of incubation, all the cells reacted negatively, whereas after 24 hours, the number of the apoptotic cells at the concentration of 10 μg proteins/ml was 3%. At the concentration of 100 μg/ml, it was as high as 15%. In the control without any antigen, it was only 2%. The results of the examinations in vitro suggest that the phenomenon of apoptosis in vivo constitutes an important defense mechanism of a parasite.