The aim of this study, was to determine the influence of the lactic acid bacteria on the growth of fungi such as Aspergillus and Rhizopus. Also the influence of the acetic acid and formic acid on the growth of the lactic acid bacteria was a subject of this research. Inhibition of microbial growth was evaluated using well plate method and measurement of changes of the impedance with the BacTrac 4300. Inhibitory in the diffusion method were metabolites of used bacteria, such as: Lactobacillus casei 0848, Lactobacillus plantarum MiLAB393, Pediococcus parvulus MiLAB099 and the mixed culture of these strains of the lactic acid bacteria (MIX), however at the measurement of the impedance a acetic and formic acids were an inhibitory (the same strain and the mixed culture). Tested strains did not have an antifungal properties. The used acids have shown inhibitory activity against the growth of selected strains of lactic acid bacteria.

Plant polyphenols thanks to the generally known health promoting antioxidant and antibacterial properties are use in the food and cosmetic industries to increase the oxidative stability of the fat-rich products and as natural antiseptics. The aim of the work was to evaluate the impact of some polyphenolic compounds in concertation not exceeding 100 µg·cm^-3 on the growth of Lactobacillus plantarum. The impact of the naringin, quercetin, gallic and ferulic acids on the bacteria growth was determined by the turbidity measurements of bacterial suspentions in microtitre plate reader Power Wave HTmicroplate. The strongest effect of poliphenols addition was observed during first 7 hours of incubation. The stimulatory effect of glycoside derivative of naringenin was dose-dependent. The addition of quercetin in 100 µg·cm^-3 concentration caused a slowdown multiplication of bacteria both in the logarithmic growth phase and in the stationary phase. The addition of phenolic acids in the tested concentrations stimulated the bacteria growth.

With every year cryopreservation is finding wider application in the storage of plant genetic resources. The number of usable, medicinal, ornamental and threatened plant species stored in liquid nitrogen is constantly increasing. The available cryopreservation techniques are becoming more and more efficient. Despite much attention is being paid to the issue of protocols optimization, the knowledge about biological material stability is still not full. The concept of cryopreservation assumes, that the stored tissues maintain their features. However, the available literature proves, that changes (mostly on DNA sequence or methylation level) can also occur. The size and durability of those alternations depends on both: the cryopreservation technique applied and the genotype. The aim of this minireview is to discuss the selected aspects of cryopreserved plants stability.