Sugar beet molasses vinasse is a by-product of ethanol fermentation from sugar beet molasses. Its dark brown colour, which is attributable to the presence of caramels, melanoidins and invert alkaline degradation products, cannot be removed by biological methods. The aim of this work was to determine the efficiency of two adsorbents haloizite clay and powder activated carbon, when used for the decolourisation of sugar beet molasses vinasse. Optimal conditions and extent of decolourisation were related to the concentration and pH of the vinasse, the time of reaction with the resin, and also to the dose and type of the resin used.

Most of known populations of microorganisms evolved adhesive abilities leading to a biofilm formation which is a unique microecosystem. This study is focused on dynamic of adhesion and biofilm formation in anaerobic conditions by the methanogenic consortium. Within this work the microscopic method enabling estimation of the number of the mathanogenic Archaea has been developed. Identification of methanogenes was based on autofluorescence of F₄₂₀ and F₄₃₀ coenzymes. Initially, the number of cells in methanogenic biofilm, was 4,4 x 10⁴ cm^-2 (at 6 h colonization), then increased to 1 x 10⁷ cm^-2 (on day 27 of colonization). Biofilm formation was faster on the plate section within the supernatant. The crucial point in biofilm formation was around 21 day, after which aggregates (microcolonies) were significantly increasing and fluorescent methanogens accumulated.

The abilities of Yarrowia lipolytica yeast to biodegradation of fatty substances in spent bleaching earth (SBE) were investigated. Yeasts inocula (of different number of propagation units) were added to SBE as free or aliginate immobilized (beads) biomass. The studied samples with SBE and yeasts were buried in soil and after winter the microbiological and chemical analyses were done as well as beads resistance and yeasts viability. The immobilized Y. lipolytica inoculum stimulated autochtonic microflora growth. The most important decrease of fatty substances in SBE was observed in presence of 50% of beads. Whereas, non depended on form and dose of yeasts was the composition of fatty acids in SBE samples, which was similar for different inocula but different from autonomous one. The effect of metabolic changes engendered by biomass suspension and immobilized cells was the decrease of contents of fatty acids of C6 – C8 and increase of C9, C16-C18. So, the inoculation of SBE with yeast could causes a specific bioconversion of fatty substances to long chain fatty acids. Noteworthy is the fact that bioremediaction was going on during winter period.